Cloning, Expression, and in Vitro Functional Activity Assay of Phic31 Integrase Cdna in Escherichia Coli

Objective: the aim of present study was cloning and expression of phic31 integrase cdna in a bacterial expression vector. thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein. materials and methods: in this experimental study, phic31 cdna was subcloned into a prokaryotic expression vector and transformed into e.coli bl21 (de3). recombinant phic31 integrase was purified form the bacterial cell lysates and its activity was verified by an in vitro functional assessment.results: sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the purified phic31 integrase confirmed the size of protein (70 kda). finally, the functionality of purified phic31 integrase was verified. conclusion: the results of this study indicated that the purified integrase has a great potential application for in vitro site-specific integration.