Effect of Embryonic Cerebrospinal Fluid on Proliferation and Differentiation of Neuroprogenitor Cells

objective: embryonic cerebrospinal fluid (e-csf) has an important role in development of embryonic and adult brain. proteomic analysis suggests that this fluid has many morphogenesandcytokinesthatalterintimeandspacethroughoutembryoniclife.theaimofthisstudywastoevaluatethedevelopmentaleffectofembryoniccsfonproliferationanddifferentiationof neuroprogenitor cells in differentgestational age.materials and methods: in this in this experimental study, we examined the role of ecsfonproliferationanddifferentiationofneuroprogenitorcellsusingneurosphereculturemethod.neurospheressizeanalysisandmttassaywereusedtoassesscellproliferationafterfour days in vitro. glial differentiation study was carried out by immunocytochemistry.neurospheres size and percentage of glial fibrialy acidic protein (gfap) positive cells were measured by image analyzer (image j). the data were analyzed by one-way anova, followed by the tukey’s post hoc test. data were expressed as mean ± sem, and differences were considered significant when p < 0.05, 0.01 and 0.001.results: viability and proliferation of neuro progenitor cells in cultures conditioned with e16 csf and e18 csf were significantly increased compare to control group. a dramatic decrease in percentage of gfap-positive cells was found following the application of csf from e16 and e18 embryos, but not e20 csf.conclusion: our data suggest that, e-csf altered proliferation and differentiation of neuro progenitorcellsinagedependentmanner.e16ande18csfenhancedproliferationandviabilityofneuroprogenitorcells,andinhibiteddifferentiationtoglialfateincomparisonwithcontrolgroup.