Three-Dimensional Scaffold From Decellularized Human Gingiva For Cell Cultures: Glycoconjugates and Cell Behavior

Objective: we studied both the presence of some carbohydrate compounds in a three-dimensional (3d) matrix harvested from human gingiva and the cell behavior in this matrix.materials and methods: in this experimental research, in order to prepare 3d scaffolds, human palatal gingival biopsies were harvested and physically decellularized by freeze-thawing and sodium dodecyl sulfate (sds). the scaffolds were placed within the rings of blastema tissues obtained from a pinna rabbit, in vitro. we evaluated the presence of glycoconjugatesand cellular behavior according to histological, histochemical and spectrophotometry techniques at one, two and three weeks after culture. one-way analysis of variance (anova)comparedthe groups.results: extracellular matrix (ecm) remained after decellularization of tissue with 1% sds. glycoconjugate contents decreased meaningfully at a higher sds concentration (p < 0.0001). after culture of the ecm scaffold with blastema, we observed increased staining of alcian blue, periodic acid-schiff (pas) and toluidine blue in the scaffold and a number of other migrant cells which was caused by cell penetrationinto the scaffold. spectrophotometry results showed an increase in glycosaminoglycans (gags) of the decellularized scaffolds at three weeks after culture.conclusion: the present study has shown that a scaffold generated from palatal gingival tissue ecm is a suitable substrate for blastema cell migration and activity.this scaffold maypotentially be useful as a biological scaffold in tissue engineering applications.