Determining Influenza Virus Shedding at Different Time Points in Madin-Darby Canine Kidney Cell Line

Objective: monitoring of influenza virus shedding and optimization of multiplicities of in-fection (moi) is important in the investigation of a virus one step growth cycle and for obtaining a high yield of virus in vaccine development and conventional basic diagnosticmethods. however, eluted infectious viruses may still be present immediately after virusinoculation and when cells are washed following virus cultivation which may lead to a falsepositive virus infectivity assay. materials and methods: in this experimental study, we investigated influenza virus progenyproductioninmadin-darbycaninekidney(mdck)cellswithfivedifferentmoiatdeterminedtimepoints.theresultswereanalyzedbyendpointtitrationtestsandimmunofluorescenceassay.results: higher titers of eluted virus were observed following a high moi inoculation ofvirus in cell culture. most probably, this was the result of sialic acid residues from viralhemagglutin in proteins that were cleaved by neuraminidase glycoproteins on the surfaceof the influenza virus, which promoted viral spread from the host cell to the culturesupernatant or during endocytosis, where viruses recycle to the cell surface by recyclingendosomes which culminated in virus shedding without replication.conclusion: we demonstrated that the pattern of influenza virus progeny production wasdose-dependent and not uniform. this production was influenced by several factors, particularlymoi. understanding the exact features of viral particle propagation has a majorimpact in producing high virus yields in the development of vaccines. use of lower moi(0.01) could result in accurate, precise quantitative assays in virus diagnosis and titrationmethods.