Evaluation of Anticancer Activity of Fruit and Leave Extracts from Virus Infected and Healthy Cultivars of Vitis Vinifera

Objective: grape virus diseases are a serious problem in iran. leaves and fruits of grapehave been used for different purposes like cooking in iran. the present investigation wascarried out to study on the cytotoxic-activities of extracts of fruits and leaves of vitis viniferafrom both virus-free and virus-infected grape cultivars against breast cancer cell line (mdamb-231)and human embryonic kidney normal cell line (hek 293).materials and methods: in this experimental study, the considered grape cultivars wereas follows: rish baba sefid, shahani ghasre shirin, rotabi zarghan, asgari najaf abad,fars, kaj angor bojnord, sarkesh shiraz and siahe zarqan. a real-time multiplex polymerasechain reaction (real-time multiplex pcr) assay was appliedto detect virus infectedcultivars.thecytotoxic effectof the methanol extractsof differentvitisvinifera varietiesonculturedcellswasmonitoredusing(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(mtt) assayat differentconcentrations (62.5, 125, 250, 500, 750, 1000 µg ml).results: among these cultivars, grapevine fanleaf virus (gflv) along with related symp-toms was detected in siahe zarqan and fars. methanolic extracts of leaves and fruits of vitisvinifera from both virus free and virus infected cultivars showed a range of limited to moderatecytotoxic activity. however, methanol extract of leaves belonged to virus infected cultivarswas found to have strong cytotoxic effect against mda-mb-231 at different concentrations. conclusion: grapevine fanleaf virus (gflv) can potentially increase the cytotoxicity ofgrape cultivars.