interference of bisphenol a on cumulus cells development and number of retrieved mature oocytes in unexpected poor ovarian response women: a prospective cohort study

Objective: this study aimed to investigate the relationship between follicular fluid bisphenol a (bpa) concentrationswith alterations in the expressions of notch1-3, caspase 3/7, hla-g, and icam-1 genes and the number ofretrieved mature oocytes (mii oocyte) in the cumulus cells of infertile poor ovarian response stimulates women.materials and methods:in this prospective cohort study, 80 infertile unexpected poor ovarian response (por) subjectswere selected on the basis of subgroup 1a of the poseidon classification. they were divided into two groups: group1 consisted of 40 women, each with a higher number of metaphase ii (mii) oocytes (g1, 3-4 oocytes retrieved), whilegroup 2 comprised of 40 women, each with a lower number of mii oocytes (g2, ≤2 oocytes retrieved). the expressionsof the studied genes were evaluated by quantitative-real time polymerase chain reaction (pcr). the concentration ofbpa in follicular fluid was measured with hplc.results: the expression levels of notch1-3, hla-g, and icam-1 genes were significantly lower in g2 than g1(p<0.05). meanwhile, caspase 3/7 expression levels were higher in unexpected por patients in g2 compared tog1 (p<0.05). there was a significant direct correlation between the levels of notch1-3, hla-g and icam-1 geneexpressions and there was also a significant inverse correlation (p<0.05) between the levels of caspase 3/7, with thenumber of mii oocytes and embryo development between the two groups. the concentration of bpa in the follicularfluids of g2 was higher compared to g1 (p<0.05).conclusion: a higher concentration of bpa was associated with a lower number of mature oocytes and oocyte qualityin these patients. also, alterations of notch1-3, caspase 3/7, hla-g, and icam-1 transcript levels in unexpectedpor women were associated with bpa concentration.