resveratrol nanoformulation inhibits invasive breast cancer cell growth through autophagy induction: an in vitro study

Objective: the aim of this study was to synthesize chitosan nanoparticles (cs nps) for resveratrol (rsv) delivery andassess their effectiveness in inducing autophagy in mda-mb 231 cells.materials and methods: in this experimental study, pure and rsv-loaded cs nps (rsv. cs nps) were prepared viathe ionic gelation method, and their physicochemical properties were characterized using standard techniques, andrsv release was measured in vitro. mda-mb 231 cells were incubated with rsv, cs nps, and rsv. cs nps andhalf-maximal inhibitory concentration (ic50) values were calculated following the mtt test. cell viability was assessedby lactate dehydrogenase (ldh) assay, and autophagy was evaluated using the real-time polymerase chain reaction(pcr).results: np formation was confirmed with the analysis of ftir spectra. pure and rsv. cs nps had 36.7 and 94.07 nmsizes with 18.3 and 27 mv zeta potentials, respectively. above 60% of rsv entrapped within nps was released in aninitial burst manner followed by a gradual release till 72 hours. cs and rsv. cs nps restrained cell proliferation at lowerconcentrations. rsv. cs nps showed the highest anticancer effect and stimulated autophagy, indicated by increasedbeclin-1 atg5, atg7, lc3a, and p62 expression.conclusion: rsv. cs nps show promising effects in inhibiting invasive breast cancer (bc) cells in vitro by inducingautophagy.