annexin a7 and its related protein suppressor of death domains regulates migration and proliferation of hca-p cells

Objective: this study was to investigate whether annexin a7 (annexina7, anxa7) and its co-related protein tumor celldeath domain silencer [suppressor of death domains (sodd)] regulates the migratory phenotype of liver cancer cells.materials and methods: in this experimental study, expression of anxa7 in hca-p cells, panxa7 downregulatedcells and panxa7 unrelated sequence cells was detected by real-time quantitative polymerase chainreaction (pcr) at mrna level and western blotting at protein level. transwell migration and invasion assayswere performed to determine the migratory phenotype.results: after inhibition of anxa7 expression, expression of sodd protein was also significantly decreased (p<0.05).transwell cell transfer experiments showed that number of tumor cells that penetrated into the cell membrane wassignificantly reduced after anxa7 silencing (p<0.05). transwell cell invasion assay showed that number of tumorcells penetrating into matrigel was significantly reduced after anxa7 down-regulation (p<0.05). the cck8 assay wasmeasured at 0, 24 and 48 hours, and proliferation rate of panxa7 lower weir cells was slower than that of hca-p cellsand panxa7 non-related sequence cells (p<0.05).conclusion: sodd expression was decreased with the down-regulation of anxa7. down-regulating anxa7 in hca-pcells decreased proliferation, migration and invasion of tumor cells.