study of the correlation between mir-106a, mir-125b, and mir-330 on multiple sclerosis patients by targeting tnfsf4 and sp1 in nf-кb/tnf-α pathway: a case-control study

Objective: multiple sclerosis (ms) is a complex multifactorial neuro-inflammatory disorder. this complexity arises from the evidence suggesting that ms is developed by interacting with environmental and genetic factors. this study aimed to evaluate the mir-106a, mir-125b, and mir330- expression levels in relapsing-remitting multiple sclerosis (rrms) patients. the mirnas' impact on tnfsf4 and sp1 genes through the nf-кb/tnf-α signaling pathway was analyzed by measuring the expression levels in case and controls. materials and methods: in this in silico-experimental study, we evaluated the association of mir-106a, mir-125b, and mir330- with tnfsf4 and sp1 gene expression levels in 60 rrms patients and 30 healthy controls by real-time polymerase chain reaction (pcr). results: the expression levels of mir-330, mir-106a, and mir125-b in blood samples of rrms patients were predominantly reduced. the expression of tnfsf4 in patients demonstrated a significant enhancement, in contrast to the diminishing sp1 gene expression level in controls. conclusion: our findings indicated an association between mir-106a and mir-330 and mir125-b expression and rrms in our study population. our data suggested that the mir106-a, mir125-b, and mir330- expression are correlated with tnfsf4 and sp1 gene expression levels.