The Effect of Mir-106b-5p Expression in the Production of Ips-Like Cells From Mice Sscs During the Formation of Teratoma and the Three Embryonic Layers

Objective: according to the mounting data, micrornas (mirnas) may play a key role in reprogramming. mir-106b is considered as an enhancer in reprogramming efficiency. based on induced pluripotent stem cells (ipscs), cell treatments have a huge amount of potential. one of the main concerns about using ipscs in therapeutic settings is the possibility of tumor formation. it is hypothesized that a procedure that can reprogram cells with less genetic manipulation reduces the possibility of tumorigenicity. materials and methods: in this experimental study, mir-106b-5p transduced by plv-mirna vector into mice isolated spermatogonial stem cells (sscs) to achieve ips-like cells. then the transduced cells were cultured in specific conditions to study the formation of three germ layers. the tumorigenicity of these ips-like cells was investigated by transplantation into male balb/c mice. results: we show that sscs can be successfully reprogrammed into induced ips-like cells by plv-mirna vector to transduce the hsa-mir-106b-5p into sscs and generating osteogenic, neural and hepatoblast lineage cells in vitro as a result of pluripotency. although these ips-like cells are pluripotent, they cannot form palpable tumors in vivo. conclusion: these results demonstrate that infection of hsa-mir-106b-5p into sscs can reprogram them into ipscs and advanced germ cell lineages without tumorigenicity. also, a novel approach for studying the generation of ipscs and the application of ips or ips-like cells in regenerative medicine is presented.