The Impact of Different Cell Culture Mediums on Cd8^+T Cells Expansion: A Bioinformatics Study

Objective: different cell culture medias can affect the expansion of t cells. the aim of this study is to assess signaling pathways, protein interactions and genes in t cells cultured in different common t cell expansion medias to select the best candidate. materials and methods: in this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis. results: the results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in srebp control of lipid synthesis, co-stimulatory signal during t-cell activation mitosis and chromosome dynamics, telomeres, telomerase, and cellular aging signal pathways. conclusion: using bioinformatics analyzes, integrated and regular genes were selected as common genes cd80, lst1, atm and itm2b 4-1bbl, akt inhibitor, interleukin 7 and 15 expansion media.