upregulation of hsa-mir-625-5p inhibits invasion of acute myeloid leukemia cancer cells through ilk/akt pathway

Objective: acute myeloid leukemia (aml) is characterized by abnormalities of differentiation and growth of primary hematopoietic stem cells (hscs) in the blood and bone marrow. in many studies, mir-625-5p has been shown to inhibit downstream pathways from affecting the metastasis and invasion of the integrin-linked kinase (ilk) signaling pathway. it has been proved that the expression of mir-625-5p decreases in aml cell lines. this study aimed to investigate the effect of mir-625-5p upregulation on the invasion of kg1 ell line in vitro. materials and methods: in this experimental study, we investigated the impact of upregulation of mir-625-5p on invasion via the ilk/akt pathway in the kg1 cell line. after transfection using the viral method, the cellular invasion was assessed by invasion assay and the levels of mir-625-5p genes and protein were evaluated by quantitative polymerase chain reaction (qpcr) and western blotting. moreover, cxcr4 level was assessed by flow cytometry. results: the invasion significantly reduced in mir-625-5p-transfected kg1 cells (p<0.01) that was concomitant with remarkably decreasing in the expression levels of ilk, nf-κb, and cox2 genes compare with the control group (p<0.01). incontrast, mmp9, ap1, and akt significantly increased (p<0.01, p<0.001 and p<0.01, respectively) and gsk3β did not change significantly in mir-625-5p-transfected kg1 cells. the protein level of nf-κb decreased (p<0.01) and mmp9 increased, however it was not significant. moreoever, the expression of cxcr4 was significantly lower (p<0.01) in comparison with the control group. conclusion: mir-625-5p leads to a reduction in cell invasion in the aml cell line through ilk pathway. therefore, it could be a breakthrough in future aml-related research. however, further studies are needed to support this argument