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proteomics analyses reveal functional differences between exosomes of mesenchymal stem cells derived from the umbilical cord and those derived from the adipose tissue
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نویسنده
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liu bin ,qiao guanglei ,cao wen ,li chenlu ,pan shaojun ,wang lirui ,liu yanlei ,ma lijun ,cui daxiang
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منبع
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cell journal (yakhteh) - 2021 - دوره : 23 - شماره : 1 - صفحه:75 -84
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چکیده
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Objective we aimed to identify the differentially expressed proteins (deps) and functional differences between exosomes derived from mesenchymal stem cells (mscs) derived from umbilical cord (uc) or adipose tissue (ad). materials and methods in this experimental study, the uc and ad were isolated from healthy volunteers. then, exosomes from ucmscs and admscs were isolated and characterized. next, the protein compositions of the exosomes were examined via liquid chromatography tandem mass spectrometry (lcms/ms), followed by evaluation of the deps between ucmsc and admsc–derived exosomes. finally, functional enrichment analysis was performed. results one hundred and ninetyeight key deps were identified, among which, albumin (alb), alphaiispectrin (sptan1), and rasrelated c3 botulinum toxin substrate 2 (rac2) were the three hub proteins present at the highest levels in the proteinprotein interaction network that was generated based on the shared deps. the deps were mainly enriched in gene ontology (go) items associated with immunity, complement activation, and protein activation cascade regulation corresponding to 24 pathways, of which complement and coagulation cascades as well as platelet activation pathways were the most significant. conclusionthe different functions of ad and ucmsc exosomes in clinical applications may be related to the differences in their immunomodulatory activities.
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کلیدواژه
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complement and coagulation cascades ,exosomes ,mesenchymal stem cells ,proteomics analysis
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آدرس
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shanghai jiao tong university, institute of nano biomedicine and engineering, shanghai engineering research center for intelligent diagnosis and treatmentinstrument, school of electronic information and electrical engineering, national center for translational medicine, collaborative innovational center for system biology, department of instrument science and engineering, china, shanghai jiao tong university school of medicine, tongren hospital, department of oncology, china, shanghai jiao tong university, bio-x institutes, key laboratory for the genetics of developmental and neuropsychiatric disorders, china, wenzhou medical university, school of laboratory medicine and life science, university town, china, shanghai jiao tong university, school of biomedical engineering, china, shanghai jiao tong university, institute of nano biomedicine and engineering, shanghai engineering research center for intelligent diagnosis and treatmentinstrument, school of electronic information and electrical engineering, national center for translational medicine, collaborative innovational center for system biology, department of instrument science and engineering, china, shanghai jiao tong university, institute of nano biomedicine and engineering, shanghai engineering research center for intelligent diagnosis and treatmentinstrument, school of electronic information and electrical engineering, national center for translational medicine, collaborative innovational center for system biology, department of instrument science and engineering, china, shanghai jiao tong university, tongren hospital, school of medicine, department of oncology, china, shanghai jiao tong university, institute of nano biomedicine and engineering, shanghai engineering research center for intelligent diagnosis and treatmentinstrument, school of electronic information and electrical engineering, national center for translational medicine, collaborative innovational center for system biology, tongren hospital, school of medicine, department of instrument science and engineering, department of oncology, china
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پست الکترونیکی
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dxcui@sjtu.edu.cn
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Authors
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