integrative analysis of lncrnas in kidney cancer to discover a new lncrna (linc00847) as a therapeutic target for staphylococcal enterotoxin tst gene

Objective: bacterial toxin can cause cell death through induction of apoptosis in cancer cell lines as well as changes in the expression patterns of long non-coding rnas (lncrnas) and genes. in the present study, the effect of tst gene on achn cell lines was reported along with proposing a novel pathway of apoptosis in kidney cancer. materials and methods: in this experimental study, effective lncrnas and genes were predicted from different criteria for renal cell carcinoma (rcc) by bioinformatics methods and lncrna-mirna-mrna interaction was constructed; then the effect of staphylococcus aureus tst gene on induction of apoptosis pathways on achn and hdf cell lines was investigated. results: after creation of lncrna-mirna-mrna interaction, changes in expression levels of lncrna linc00847 (p=0.0024) and pten gene (p=0.0027) were identified, as potential apoptosis biomarkers for kidney cancer, after treating achn cell line by pcdna3.1 (+)-tst compared to the empty vector. in contrast, there was no statistically significant difference in dicer1 expression levels in achn-tst cell (p≥0.05). in addition, transfection by pcdna3.1 (+)-tst could increase achn cell apoptosis level (p<0.0001) compared to the pcdna3.1 (+) group; but no significant effect was observed on normal cells. conclusion: it is suggested that lncrna linc00847, discovered in this study, could provide a new landscape for researches aimed to determine relationship between functional lncrna and rcc pathways. pcdna3.1 (+)-tst was found to increase apoptosis in the transfected cells.