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Evaluation of Bcl-2 Family Gene Expression in Hippocampus of 3, 4-ethylenedioxymethamphetamine Treated Rats
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نویسنده
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Soleimani Asl Sara ,Farhadi Mohammad Hassan ,Mousavizadeh Kazem ,Samadikuchaksaraei Ali ,Soleimani Mansoure ,Jameie Behnamedin ,Joghataei Mohammad Taghi ,Samzadeh-Kermani Alireza ,Hashemi-Nasl Hamed ,Mehdizadeh Mehdi
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منبع
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cell journal (yakhteh) - 2012 - دوره : 13 - شماره : 4 - صفحه:275 -280
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چکیده
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Objective: 3,4-methylenedioxymethamphetamine (mdma) is an illicit, recreational drug that causes cellular death and neurotoxicity. this study evaluates the effects of different doses of mdma on the expression of apoptosis.related proteins and genes in the hip- pocampus of adult rats. materials and methods: in this expremental study,a total of 20 male sprague dawley rats (200-250 g ) were treated with mdma (0, 5, 10, 20 mg/kg i.p. twice daily) for 7 days. seven days after the last administration of mdma, the rats were killed. bax and bcl-2 genes in addition to protein expressions were detected by western blot and reverse transcription- polymerase chain reaction (rt-pcr).results were analyzed using one-way anova and p.0.05 was considered statistically significant. results: our results showed that mdma caused dose dependent up-regulation of bax and down-regulation of bcl-2 in the hippocampus. there was a significant alteration in bcl-2 and bax genes density. conclusion: changes in apoptosis-related proteins and respective genes relating to bax and bcl-2 might be involved in the molecular mechanism of mdma-induced apoptosis.
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کلیدواژه
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4-methylendioxymethamphetamine (MDMA) ,Apoptosis ,Bcl-2 ,Bax
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آدرس
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hamadan university of medical sciences, Department of Anatomical Sciences, ایران, university of social welfare and rehabilitation sciences, Substance Abuse and Dependence Research Center, ایران, tehran university of medical sciences tums, Cellular and Molecular Research Center, ایران, tehran university of medical sciences tums, Cellular and Molecular Research Center, ایران, tehran university of medical sciences tums, Cellular and Molecular Research Center, ایران. tehran university of medical sciences tums, Department of Anatomical Sciences, ایران, tehran university of medical sciences tums, Department of Anatomical Sciences, ایران, tehran university of medical sciences tums, Cellular and Molecular Research Center, ایران, zabol university, Faculty of Sciences, Department of Chemistry, ایران, tehran university of medical sciences tums, Department of Anatomical Sciences, ایران, tehran university of medical sciences tums, Cellular and Molecular Research Center, ایران. tehran university of medical sciences tums, Department of Anatomy, ایران
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پست الکترونیکی
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mehdizadehm@tums.ac.ir
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Authors
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