A Study of the Major Components of the PluriNet Network Expression in Dental Pulp Stem Cells Isolated from Human Third Molar Teeth

Objective: the objective of this study was to isolate and culture human dental pulp stem cells to study important stem cell markers in them. materials and methods: dental stem cells were isolated from human pulp and cultured in alpha-modified eagle’s medium (α-mem) supplemented with 20% fetal bovine serum (fbs) in a 37°c incubator with 5% co2 and photographed under inverted microscope. the expressions of the important stem cell markers were analyzed by reverse transcription polymerase chain reaction (rt-pcr) and agarose gel electrophoresis in the cells at different passages. results: cells isolated from dental pulp showed a high rate of proliferation and were cultured to more than 15 passages in vitro. the study of gene expression by rt-pcr showed that these cells expressed nucleostemin, cyclin d1, oct-4 and nanog (major components of the plurinet) in different passages as well as under serum-free conditions. conclusion: cells isolated from dental pulp are genuine pluripotent stem cells with high potential for self-renewal. the expression of the stem cell markers in human dental pulp stem cells indicate that they have a great potential for cell therapy and regenerative medicine, even they were isolated from adult teeth.