Proteome Analysis of Mouse Brain Exposed to Chronic Hypoxia

Objective: chronic hypoxia exists in many diseases, including cancer. the subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain. materials and methods: using the empai protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7% o2 for 48 hours. results: utilizing the empai protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression. conclusion: deregulated proteins were mainly involved in cell metabolism, apoptosis, ca2+ signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. the obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.