panax ginseng extract improves follicular development after mouse preantral follicle 3d culture

Objective: panax ginseng is a popular traditional herb that has been used in complementary and alternative medicine in eastern asia, and it possesses pharmacologically active compounds like ginsenosides (gss). this study aimed to investigate the impact of panax ginseng extract (pge) at different concentrations on in vitro follicular function and development in a three-dimensional (3d) culture system fabricated using sodium alginate after 12 days of culture. materials and methods: in this experimental study, preantral follicles (n=661) were mechanically isolated from the ovaries of 14-day-old female nmri mice using 29-gauge insulin syringes. follicles were individually capsulated within sodium alginate, and divided into four groups including control and experimental groups 1, 2, and 3. then, they were cultured for 12 days in the medium supplemented with different concentrations of pge (0, 50, 100, and 500 μg/ ml, for control groups and groups 1, 2 and 3, respectively). at the end of the culture period, the mean diameter and maturation of follicles, follicular steroid production, mrna expression level of proliferating cell nuclear antigen (pcna) and follicle stimulating hormone receptor (fsh-r), and reactive oxygen species (ros) levels in collected metaphase-ii (mii) oocytes were determined. results: the mean diameter of follicles in group 2 was significantly increased as compared to other groups (p<0.001). the percentages of the survival and maturation rate and levels of secreted hormones were higher in group 2 than the other groups (p<0.05). follicles cultured in the presence of pge 100 μg/ml had higher levels of proliferation cell nuclear antigen (pcna) and follicle stimulating hormone receptor (fsh-r) mrna expression in comparison to other groups (p<0.05). moreover, oocytes collected from groups 2 and 3 had lower levels of ros as compared to other groups (p<0.05). conclusion: our results suggest that pge at the concentration of 100 μg/ml induces higher follicular function and development in the 3d culture system.