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design and fabrication of a microfluidic chip for oocyte denudation.
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نویسنده
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safaefar f. ,karamdel j. ,veladi h. ,fallah tafti m. ,maleki m.
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منبع
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مهندسي برق دانشگاه تبريز - 1401 - دوره : 52 - شماره : 3 - صفحه:189 -194
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چکیده
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Intracytoplasmic sperm injection (icsi) is one of the most successful techniques of assisted reproductive technology (art) and is mostly in use for the treatment of infertility with male factors. in this method, before injecting sperm into the intracytoplasmic of the oocyte, cumulus cells around the oocyte must be stripped to facilitate the injection process. to achieve this, both enzymatic and mechanical methods are used in embryological laboratories for denudation, which has major deficiencies, including the possibility of damaging the oocyte prior to the injection process. in this research, a microfluidic-based device is introduced for the separation of cumulus cells around the oocyte with minimum manual operations. the results prove high efficiency, and non-destructive denudation of the oocyte with the reduced amount of culture medium leads to the low-cost preparation process of oocytes. the process can also be integrated with icsi chips under development and will be reported shortly.
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کلیدواژه
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assisted reproductive technology (art) ,denudation ,in vitro maturation (ivm) ,microfluidics ,lab-on-chip
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آدرس
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islamic azad university south tehran branch, faculty of technical and engineering, biomedical engineering department, iran, islamic azad university south tehran branch, faculty of technical and engineering, electrical engineering department, iran, university of tabriz, faculty of electrical and computer engineering, microsystem fabrication laboratory, iran. islamic azad university south tehran branch, faculty of technical and engineering, biomedical engineering department, iran, islamic azad university south tehran branch, faculty of technical and engineering, biomedical engineering department, iran, islamic azad university tabriz branch, department of biology, iran. islamic azad university south tehran branch, faculty of technical and engineering, biomedical engineering department, iran
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پست الکترونیکی
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m_fallah@azad.ac.ir
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design and fabrication of a microfluidic chip for oocyte denudation.
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Authors
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safaefar f. ,karamdel j. ,veladi h. ,fallah tafti m. ,maleki m.
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Abstract
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intracytoplasmic sperm injection (icsi) is one of the most successful techniques of assisted reproductive technology (art) and is mostly in use for the treatment of infertility with male factors. in this method, before injecting sperm into the intracytoplasmic of the oocyte, cumulus cells around the oocyte must be stripped to facilitate the injection process. to achieve this, both enzymatic and mechanical methods are used in embryological laboratories for denudation, which has major deficiencies, including the possibility of damaging the oocyte prior to the injection process. in this research, a microfluidic-based device is introduced for the separation of cumulus cells around the oocyte with minimum manual operations. the results prove high efficiency, and non-destructive denudation of the oocyte with the reduced amount of culture medium leads to the low-cost preparation process of oocytes. the process can also be integrated with icsi chips under development and will be reported shortly.
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Keywords
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assisted reproductive technology (art) ,denudation ,in vitro maturation (ivm) ,microfluidics ,lab-on-chip
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