cryopreservation of spermatogonial stem cells of native goat of iran

This study deals with cryopreservation of spermatogonial stem cells (sscs) of native goat using different media along with cryoprotectant. morphological differentiation and immunocytochemistry tests were used to identify the cells. furthermore anti-vimentin and anti-oct-4 immuno staining methods were used for identification of sertoli cells and sscs, respectively. cryopreservation of sscs was done with two sets of media. one with 40% dulbecco’s modified eagle’s medium (dmem), 50% fetal bovine serum (fbs) and 10% dimethyl sulfoxide (dmso). the second medium included 90% fbs and 10% dmso. post thaw cryopreserved cells were subjected to viability and colony area formation on 4^th, 8^th and 12^th days of cultur-ing after and before cryopreservation. results clearly indicated the viability, area and number of colonies in the first medium registered to 61.37%, 0.87 mm^2 and 246.88 averagely in every 25 cm^2 culture flask, respec-tively. similarly with second medium, post thaw cryopreserved sperm registered viability, area and number of colonies to 73.87%, 2.74 mm^2 and 364.36 in every 25 cm^2 culture flask, respectively. after the thawing of spermatogonial cells, the best viability percentage was obtained in the freezing medium containing 90% fbs. thus the study demonstrated that serum concentration had a distinct positive effect on the mainte-nance and proliferation of sscs in culture after cryopreservation.