effect of agar and different culture media on the micropropagation of rosa hybrida cv.’black baccara’

In vitro propagation of plant has played a very important role in rapidmultiplication of cultivars with desirable traits and production of healthy anddisease-free plants. in the present investigation, the objectives were to optimizethe micropropagation of rose hybrid ‘black baccarat’ cultivar. in proliferationstep, the nodal segments (1.5 cm) was cultured on both liquid and solid media(ms, vs and wpm). the results showed that the highest shoot proliferationwas obtained on vs medium. the highest amount of multiplication and thegrowth rate were obtained in the liquid medium. for rooting, three concentrationsof vs mineral salts (full-, half-, and quarter-strength) containing naa (0.5?m) were tested in semi-solid and liquid media. root initiation influenced bymineral concentration in the medium. the investigation showed that thehighest number of roots was observed in semi-solid 1/4 vs medium. variationin multiplication and growth rate of explants can be explained on the basis ofwater potential and mineral availability to the explants in the liquid medium.