indirect shoot regeneration in anthurium andreanum ‘clisto’ from leaf explant

Anthurium is commercially produced as cut flowers and potted plantsand known for its colorful spathes. it is traditionally propagated by stem cuttingand suckers, so micropropagation is an alternative production method. in thisexperiment we carried out shoot regeneration in anthurium andreanum ‘clisto’.the leaf explant was used and after sterilization, they cultured on two differentmedia (half-strength ms and nitsch’s media) which supplemented with differentcompositions of 2,4-d (0, 0.25, 0.5 and 0.75 mg/l) and ba (0, 0.5, 1 and 1.5mg/l). after three months callus size and percentage of explant that producedcallus were measured and callus transferred to ms media with different concentrationsof ba (0, 0.25, 0.5 and 0.75 mg/l). after 6 months, number of shoot,shoot length and leaf number was recorded. the highest callus proliferation wasobtained in nitsch’ media supplemented with 0.25 mg/l 2,4-d and 0.25 or 0.5mg/l ba. the callus was creamy color and compact. the highest shootregeneration was observed in media with 0.75 mg/l ba. we cloud achieved 31shoot per callus segment in the best treatment. rooting was easily performed inpeat: perlite (1:2) pots and they well acclimated and transferred to greenhouseand 99% plants were survived.