Plantlet Regeneration through Indirect Organogenesis of Flame Gold Tree (Koelreuteria elegans Laxm.)

Koelreuteria elegans, popularly known as “flame gold” is an ornamentaltree. in vitro callus induction and regeneration from various explants (eafsegments and cotyledonary leaf) were studied on modified ms medium. thehighest callus induction rate (80%) and multiplication was obtained in 2 mg/l2,4-d from leaf segments. calli transferred in 1.5 mg/l bap resulted inefficient shoot regeneration (70%) and development (4.35 shoots). ms halfstrength medium supplemented with 0.2 mg/l naa reported 80% rooting after21 days of implantation. mostly, the roots were long and healthy. plants weresuccessfully transferred in sterilized mixture of vermiculite: soil: sand (3:1:1)with 65% survival rate under field conditions. the in vitro regenerated plantletswere hardened and acclimatized successfully.