Some Useful Information About Micropropagation

This review article investigates a comprehensive description of thefactors, methods, strategies, approaches and prerequisites influencing in vitromicropaopagation and the amount of their use across the world. the findingspresented here are the results of the study of several hundred papers publishedall over the world. tissue culture techniques are routinely used for micropropagation.enhancement of efficiency and efficacy of plant regeneration are primary goalsof micropropagation. in this article, maximum and minimum use of some basicissues concerned with plant tissue culture in vitro especially micropropagationsuch as types of explants, types of culture media, types of sterilizing agent forexplants, types of plant growth regulators (pgrs) for general studies of miropropagation,shoot and root induction, somatic embryogenesis (se) and callusinduction have been considered. maximal application for explants, culturemedia and sterilizing agent are single node, murashige and skoog (ms) andsodium hypochlorite (naclo), respectively. ba and naa are the mostapplication among cytokinins (cks) and auxins for general studies of miropropagation.among all pgrs used for general studies of miropropagation, naa ison the top. ba and iba are the most use among cks and auxins for shootingand rooting of explants, respectively. 2,4-d, naa and tdz are used more thanthe other pgrs for induction of se. among all types of auxins and cks used assingular or in combination with them for callus induction, 2,4-d is at the toplevel. combination of ba and naa is the maximum for general studies ofmiropropagation. this review article can help to the future studies on micropropagationdue to the correct selection of the treatments.