In Vitro Flower Bud Formation, Plant Regeneration and Morphogenetic Studies in Local Scented Cultivar of Rosa indica

In vitro propagation of rose (rosa indica) as well as rose breeding is avaluable economic enterprise and has received highest importance globallyincluding indian subcontinent. the development of new cultivars for commerceis faced with new challenges. in vitro micropropagation protocols of varietiesof roses have been established but very few reports on the clonal propagationof local cultivars of indian scented rose has been made. in the present paperwe report the dedifferentiation and redifferentiation of stem nodal, leaf disc,petals well as ovary explants cultured on ms medium supplemented withvarious growth adjuvants such as coconut water (cw 5%-20%v/v), auxins(iaa, naa, 2,4-d and iba) and cytokinins (bap and kn) in varying concentrations.vigorous callus formation was observed showing embryogenicclusters in both the nodal and leaf disc explants on ms medium supplementedwith cw(10% v/v) + 2,4- d (11.3 ?m) + bap (3.55 ?m). the proliferationof 4-7 multiple shoots (mean number of shoots 5 ± 1.24) as well as in vitroflower bud formation on in vitro regenerated rose plants from nodal explantscultured on ms medium supplemented with cw + 2,4-d + bap have beenobserved. the regenerated shoots treated with ‘quic root’ (commerciallyavailable rooting chemical) produced well developed root system.