An Efficient and Cost Effective Protocol for in Vitro

An efficient and cost effective protocol for in vitro propagation ofpineapple (ananas comosus var. queen) has been developed. in the proliferationstage, agar based murashige and skoog (ms) media was supplemented with3.0 mg/l benzyleaminopurine (bap), 0.5 mg/l indole acetic acid (iaa) and 50mg/l adenine sulphate as rbc design experiment. two approaches were takento reduce the chemical cost of micropropagation media. analytical gradesucrose was successfully replaced by commercial sugar, completely duringproliferation stage and up to 66% during rooting stage. again during therooting stage, agar based solid media was replaced by liquid media (msmedia).bio-degradable coir and luffa were used as supporting matrix. as supportingmatrix in rooting media, luffa was found to be more effective. theclonal fidelity of in vitro raised plantlets was confirmed by rapd technique.