Evaluation of Main Anthocyanins in Skin of Forest Pear (Pyrus Pyraster) (Sange Khouj) Fruit in Guilan Province of Iran

The anthocyanins of the fruit skin of iranian forest pear (sange khouj)were separated, identified and quantified using high-performance liquid chromatography/ diode array detection (hplc / dad) and hplc / electrosprayionization / mass spectrometry (hplc / esi / ms). the individual anthocyaninswere identified by comparing their mass spectral data and retention times withthose of standards and published data. cyanidin-3-galactoside was the majorcompound, taking up 93% of the total anthocyanin content. the content ofcyanidin-3-galactoside reached 23.7 ± 3.2 mg 100 g-1 fresh weight, followedby the eighth peak (2.14%) and cyanidin-3-glucoside (1.23%). pelargonidin-3-rutinoside was identified for the first time in ordinary pear (p. communis l).dpph (2, 2-diphenyl-1-picrylhydrazyl) and frap (ferric reducing antioxidantpower) assays showed that the extract from the fruit peel of ‘sange khouj’contained anthocyanins and possessed high antioxidant capacity.