Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong Clay

In this paper,immobilization of multi-enzyme using calcium alginate-clay beads for tapioca slurry saccharification process was carried out. the kinetics parameter and immobilization yield for alpha-amylase,glucoamylase,cellulase and multi-enzyme system were compared. the michaelis constant,km for free alpha-amylase,glucoamylase,cellulase and multi-enzyme were 2.0831,1.8326,7.8592 and 3.5367 mg/ml,respectively. as for the immobilized system,the km values were 3.1604,2.1708,9.2791 and 4.91176 mg/ml,respectively. the km value for the immobilized multi-enzyme system was 1.39 times higher than free multi-enzyme system. this suggests that the immobilized multi-enzyme is a potential system for tapioca saccharification process due to affinity of enzyme to the substrate. for the immobilization yield,the yield for alpha-amylase,glucoamylase,cellulase and multi-enzyme were 51.19,50.21,40.68 and 53.71 %,respectively. the result showed the immobilized multi-enzyme had a higher yield compared to the single enzyme which could be explained due to the synergistic action of alpha-amylase,glucoamylase and cellulose enzymes. therefore,it can be concluded that the use of immobilized multi-enzyme system had a very good potential for starch bioconversion into glucose. © 2014 faculty of mechanical engineering.