effect of interferon- on the expression of regulatory nlrp6 and nlrp12 genes in human wharton’s jelly mesenchymal stem cells

Background: mesenchymal stem cells (mscs) are well-known for their immune-modulatory properties. a subgroup of the nucleotide-binding oligomerization domain (nod)-like receptors (nlrs) has been recently identified to play a regulatory role in immune and inflammatory responses. this study aims to analyze and compare the gene expression levels of the nod-like receptor family pyrin domain-containing proteins (nlrps), such as nlrp6 and nlrp12, in wharton’s jelly-derived mesenchymal stem cells (wj-mscs) treated with interferon-gamma (ifn-γ), the pro-inflammatory cytokine, and untreated cells.methods: the immunophenotypic characterization of the isolated wharton’s jelly mesenchymal stem cells (wj-mscs) was conducted using flow cytometry. next, they were cultured with or without ifn-γ, followed by a comparison of the expression levels of the nlrp6 and nlrp12 genes using quantitative pcr (qpcr).results: treatment of cells with ifn-γ resulted in a statistically significant increase in nlrp12 gene expression compared to untreated cells. in contrast, the expression of nlrp6 did not differ significantly between cells with or without ifn-γ treatment.conclusion: the altered expression level of nlrp12 suggests its potential role in the inflammatory regulation mediated by wj-mscs in response to ifn exposure; however, further studies are needed to validate its role in experimental models of inflammatory-related diseases.