detection and identification of prevotella melaninogenica in saliva samples of patients with ulcerative colitis

Background: ulcerative colitis (uc) is a long-term inflammatory bowel disease (ibd) caused by abnormal immune responses, leading to inflammation and scarring in the large intestine. the bacteria prevotella melaninogenica, found in the intestine and mouth, may contribute to uc. this study focuses on the detection of p. melaninogenica in the saliva of uc patients and compares them with the healthy control (hc) group.methods: the present study was a case-control study including 40 uc patients and 40 healthy controls (hcs) with an average age of 43.0 3± 10.3. this study used a real-time pcr test to investigate the frequency and average number of p. melaninogenica from the 16s rrna gene sequence of p. melaninogenica in both groups.results: p. melaninogenica was more frequent in uc patients (77.5%) than hcs (45%) (p = 0.003). the patient group had more bacteria (339.31 ± 1082.29) than hcs (61.29 ± 154.03) (p = 0.005). women in the uc group had more p. melaninogenica (492.35 ± 1427.61) than the control group (56.98 ± 123.50) (p = 0.0342). similarly, men in the uc group (262.85 ± 664.97) had more bacteria than the control group (72.62 ± 222.76) (p = 0.015).conclusion: the current study showed that dysbiosis in p. melaninogenica, a bacterium in human saliva, could be important in the development of uc. further investigation is needed to evaluate its use as a potential biomarker in the uc.