nickel nanoparticles/recycled polyethylene terephthalate nanofibers reduce algd expression in pseudomonas aeruginosa

Background and objectives: recycled polyethylene terephthalate (rpet) nanofibers have become an important part of human life, with a continuous increase in their production and consumption. herein, the antibacterial activity of nickel nanoparticles/recycled polyethylene terephthalate nanofibers (ninp/rpet nf web) was evaluated by analyzing alginate expression in pseudomonas aeruginosa, as an opportunistic microorganism. methods: ninps were synthesized and ninp/rpet nf was produced by adding 25 μg/ml of ninp to 10% solutions of rpet at a weight ratio of 3%. after exposing p. aeruginosa (pa01) to ninp/rpet nf, the biofilm-forming capacity was determined and real-time pcr was performed to measure algd expression. results: treatment with 25 μg/ml of ninp/rpet nf reduced growth of p. aeruginosa on mueller hinton agar but did not result in complete inhibition. the biofilm optical density (550 nm) was 0.464 ± 0.021 after treatment with ninp/rpet nf and 0.082± 0.011 in the absence of ninp/rpet nf. this indicates the significant reduction of biofilm formation after exposure to ninp/rpet nf (p=0.01). in addition, a 0.6-fold (p=0.03) reduction in alginate expression was detected by real-time quantitative real-time pcr. conclusion: our results indicate the potential of ninp/rpet nf for application in nano-based antibacterial medical systems.