synthesis of new three-component derivatives of 1, 3, 4-oxadiazole and evaluation of their in vitro antibacterial and antifungal properties

Background and objectives: antibiotic resistance is a major public health challenge. the pervasive antibiotic misuse can lead to increased antibiotic resistance. thus, there is a need for discovery of new compounds against drug-resistant microorganisms. we synthesized new series of 1, 3, 4-oxadiazole derivatives (4a-4d) and evaluated the antibacterial and antifungal activity of the derivatives against staphylococcus aureus, staphylococcus epidermidis, acinetobacter baumannii, klebsiella pneumoniae, aspergillus fumigatus and aspergillus flavus. methods: the new derivatives of 1, 3, 4-oxadiazole were synthesized using a single-stage, high-yield method. the structure of the new compounds was confirmed by infrared spectroscopy, carbon-nuclear magnetic resonance and hydrogen- nuclear magnetic resonance. then, antibacterial and antifungal activities of the prepared derivatives (1 mg/ml) were evaluated by determining minimum inhibitory concentration and minimum bactericidal/fungicidal concentration using the agar well diffusion method. results: all synthesized compounds, especially (4d) with methoxyphenyl group, exhibited powerful antibacterial activity against the tested bacteria. however, the compounds had no antifungal effect. conclusion: our findings indicate the antibacterial potential of the novel synthetic 1, 3, 4-oxadiazole compounds.