COMPARISON OF LIPOAMIDE DEHYDROGENASE ACTIVITY IN HL-60 LEUKEMIA CELLS AND NORMAL LYMPHOCYTE

To determine the importance of q10h2 as an antioxidant in cancer, we measured the activityof lipoamide dehydrogenase (q10h2 recycling enzyme) in hl-60 and normal lymphocyte. the culturedcells of hl-60 and human normal lymphocytes were assayed in cell lysate of given number of both hl-60 and normal lymphocyte. the activity of lipoamide dehydrogenase and the protein concentration weredetermined by spectrometric methods. the activity of lad was found to be 0.216 mu mol/min/ىg proteinin hl-60 cells and 0.0415mu mol/min/ىg protein in normal lymphocytes. although the average proteinconcentration ratio in hl-60 cells to normal lymphocyte was found to be %124, the average ratio ofenzyme activity in hl-60 to normal lymphocyte was %526. these results are indicative ofindependence of enzyme increase in hl-60 cells of increase of protein synthesis.