Saturated Picric Acid Prevents Autophagia and Self-Mutilation in Laboratory Rats

The dysesthesia and paresthesia that occurs in laboratory rats after spinal cord injury (sci)results in autophagia. this self-destructive behavior interferes with functional assessments in designedstudies and jeopardizes the health of the injured rat. in this study, we evaluated role of saturated picricacid in the prevention of autophagia and self-mutilation. all rats were anesthetized with anintraperitoneal injection of a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg) for the sciprocedures. in the first 39 rats, no solution applied to the hind limbs, but in the next 26 cases, wesmeared the saturated picric acid on the tail, lower extremities, pelvic, and abdomen of the ratsimmediately after sci. in the rats without picric acid, 23 rats died following autophagia, but in the 26rats with picric acid, there was no autophagia (p < 0.001). picric acid side effects in skin andgastrointestinal signs such as irritation, redness and diarrhea were not seen in any rat. saturated picricacid is a topical solution that if used appropriately and carefully, might be safe and effectively preventsautophagia and self-mutilation. when the solution is applied to the lower abdomen and limbs, wepresume that its bitterness effectively prevents the rat from licking and biting the limb.