Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking

In this study,our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results,we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented with 10% fetal bovine serum and characterized by standard methods. moreover,karyotyping was performed to evaluate chromosomal stability. mean of donors’ age was 47.6 ± 8.29 year,mean of cell viability was 95.6 ± 1.51%,and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. the main aim of this project was demonstrating the feasibility of cgmp-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials. © 2015 tehran university of medical sciences. all rights reserved.