Evaluation of the Expression of P-Glycoprotein in Propoxur-Resistant Caco-2 Cells

There is a great concern about the effect of propoxur, as one of the more common n-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. caco-2 cells became resistant to propoxur, and the resistance was confirmed through mtt assay. then the cell membrane integrity and p-glycoprotein expression were measured by ldh assay and western blot analysis, respectively and compared to the parent cells. contrary to what was expected, the expression of p-glycoprotein in propoxur resistant cells was lower than parent cells. conclusion: this study indicates that the resistance to propoxur may not be related to p-glycoprotein expression directly, since pglycoprotein expression has decreased in these cells.