DETECTION OF HUMAN PAPILLOMAVIRUS DNA SEQUENCES IN ORAL LESIONS USING POLYMERASE CHAIN REACTION

The purpose of the present study was to estimate the frequency of hpv dna in four groupsof oral lesions, including oral squamous cell carcinoma. sixty paraffin-embedded oral tissue sampleswere examined for the presence of hpv dnas using the pcr technique. these specimens wereobtained from patients with oral squamous cell carcinoma (oscc), leukoplakia, oral lichen planus(olp), and pyogenic granuloma (pg). consensus primers for l1 region (my09 and my11) andspecific primers were used for detection of hpv dna sequences in this study. we detected hpv dnain 60% (9 out of 15) of osccs, 26.7% (4 out of 15) of leukoplakia, 13.3% (2 out of 15) of olps, and6.7% (1 out of 15) of pgs. statistical analysis showed that the prevalence of hpv in oscc wassignificantly higher than other groups (p < 0.05). the frequency of hpv-16 and 18 detection in osccsamples were 40% and 20%, respectively. the prevalence of these high risk hpvs was significantlyhigher in oscc group (p < 0.05). the results of the present study show a successive increase ofdetection rate of hpv-16 and 18 dnas from low level in samples of pyogenic granuloma and nonpremalignantor questionably premalignant lesions of olp to premalignant leukoplakia and to oscc