Dendritic Cell Stimulation by IFN-β Alters T Cell Function via Modulation of Cytokine Secretion in Diabetes Type 1

During antigen capture and processing, mature dendritic cells (dc) express large amounts of peptide-mhc complexes and accessory molecules on their surface. we investigated the role of ifn-β in induction hla-g expression on the monocyte derived dc and cytokine profile in diabetes type 1. we accomplished secretary pattern and total cytokine production of the th1 cytokine (il-2, γifn) and th2 cytokines (il-4, il-10) before and after mixed leukocyte reaction (mlr) of 30 diabetic patients and 30 normal subjects. in this study a significant increase of il-10 and γifn reduction after ifn-β therapy in culture in presence of hla-g bearing dc as compared to control were seen. it is seen that dendritic cell causes il-10 production of t cell in vitro that reduce t cell activation from diabetes patients and normal subjects resulted to the production and expression of hla-g on these cells from both groups. using mixed leukocyte reaction, it was found that ifn-β-treated dendritic cell mediated the inhibition of autologous t cell activation via il-10 production and level of hla-g on dendritic cell may be correlated to disease activity in diabetes patients and it could also serve as a useful marker for disease progress and treatment.