Anti-mutagenic and Pro-apoptotic Effects of Apigenin on Human Chronic Lymphocytic Leukemia Cells

Diet can play a vital role in cancer prevention. nowadays the scientists are looking for food materials which can potentially prevent the cancer occurrence. the purpose of this research is to examine anti-mutagenic and apoptotic effects of apigenin in human lymphoma cells. in present study human chronic lymphocytic leukemia (eheb cell line) were cultured in rpmi 1640 (sigma), supplemented with 10% fetal calf serum, penicillin-streptomycin, l-glutamine and incubated at 37 ؛c for 2 days. in addition cancer cell line was treated by and apigenin and cellular vital capacity was determined by mtt assay. then effect of apigenin in human lymphoma b cells was examined by flow cytometry techniques. the apigenin was subsequently evaluated in terms of anti-mutagenic properties by a standard reverse mutation assay (ames test). this was performed with histidine auxotroph strain of salmonella typhimurium (ta100). thus, it requires histidine from a foreign supply to ensure its growth. the aforementioned strain gives rise to reverted colonies when expose to sodium azide as a carcinogen substance. during mtt assay, human chronic lymphocytic leukemia revealed to have a meaningful cell death when compared with controls (p<0.01) apoptosis was induced suitably after 48 hours by flow cytometry assay. in ames test apigenin prevented the reverted mutations and the hindrance percent of apigenin was 98.17%.these results have revealed apigenin induced apoptosis in human lymphoma b cells in vitro.