ASSOCIATION BETWEEN THE SEVERITY OF ANGIOGRAPHIC CORONARY ARTERY DISEASE AND PARAOXONASE-1 PROMOTER GENE POLYMORPHISM T(-107)C IN IRANIAN POPULATION

The oxidation of low-density lipoproteins and cell membrane lipids is believed to play an integral role in the development of fatty streak lesions, an initial step in coronary artery disease (cad). paraoxonase-1 (pon1) is an enzyme associated with the high-density lipoprotein (hdl) particle. pon1 protects ldl from oxidative modification by hydrolyzing lipid peroxides, suggestive of a role for pon1 in the development of cad. the present study tested the hypothesis that paraoxonase-1 promoter polymorphism t(-107)c could be a risk factor for severity of cad in iranian population. paraoxonase-1 promoter genotypes were determined in 300 consecutive subjects (> 40 years old) who underwent coronary angiography (150 subjects with >50% stenosis served as cases [cad+] and 150 subjects with < 20% stenosis served as controls [cad-]). pon1 promoter genotypes were determined by pcr and bstu1 restriction enzyme digestion. cad+ subjects did not show any significant differences in the distribution of pon1 promoter genotypes as compared to cad- subjects (p = 0.075). however the analysis of pon1 promoter genotypes distribution showed a higher percentage of (-107) tt among cad+ compared with cad- (p = 0.027). after controlling for other risk factors, the t(-107)c polymorphism had interaction with age (p = 0.012), but did not show any interaction with other risk factors such as bmi ,gender, smoking, diabetes, level of hdl-c, ldl-c, triglyceride and total cholesterol. these data suggest that the tt genotype may represent a genetic risk factor for coronary artery disease in iranian population