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   Immobilization of Laccase From Trametes Hirsuta Onto Cmc Coated Magnetic Nanoparticles  
   
نویسنده Sadeghzadeh S. ,Ghazvini S. ,Hejazi S. ,Yaghmaei S. ,Ghobadi Nejad Z.
منبع International Journal Of Engineering - 2020 - دوره : 33 - شماره : 4 - صفحه:513 -519
چکیده    In this study, fe3o4/cmc magnetic nanoparticles were synthesized through co-precipitation method. afterward, laccase from trametes hirsuta was immobilized onto carboxymethyl cellulose (cmc)-coated magnetic fe3o4 nanoparticles by covalent bonding between carboxyl groups of carboxymethyl cellulose and amine group of laccases. also, the resulted magnetic nanoparticles and immobilized laccase were characterized by fourier-transform infrared spectroscopy (ftir), scanning electron microscope (sem) and dynamic light scattering (dls) analysis. moreover, the vital factors in enzyme immobilization, such as contact time, amount of n-hydroxysuccinimide (nhs), and the amount of nanoparticles were optimized, which successively 48 h, 0.01 g, and 0.0125 g were achieved for 0.01g of n-ethyl-n′-(3-dimethylaminopropyl)carbodiimide (edc). activity recovery of 51 ± 0.8% was achieved by optimizing the immobilization process. the results also indicated that the loading of laccase onto carboxymethyl cellulose-coated fe3o4 nanoparticles was approximately 120 (mg/g). finally, the immobilized laccases on magnetic support could save nearly 50% of their initial activity after five consecutive cycles.
کلیدواژه Laccase ,Immobilization ,Fe3o4/Cmc ,Trametes Hirsuta
آدرس Sharif University Of Technology, Biochemical & Bioenvironmental Research Center, Chemical & Petroleum Engineering Department, Iran, Sharif University Of Technology, Chemical & Petroleum Engineering Department, Iran, Sharif University Of Technology, Chemical & Petroleum Engineering Department, Iran, Sharif University Of Technology, Chemical & Petroleum Engineering Department, Iran, Sharif University Of Technology, Biochemical & Bioenvironmental Research Center, Iran
پست الکترونیکی z_ghobadi@che.sharif.edu
 
     
   
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