|
|
Hemp Fibres As Novel Green Support Material For Immobilization of Acidithiobacillus Ferrooxidans
|
|
|
|
|
نویسنده
|
Akhlaghi N.
|
منبع
|
International Journal Of Engineering - 2019 - دوره : 32 - شماره : 9 - صفحه:1225 -1230
|
|
|
چکیده
|
The suitability of hemp fibres as green support material for immobilization of acidithiobacillus ferrooxidans cells in ferrous iron bio-oxidation process was investigated. a series of experiments were carried out in shake flasks and bioreactor for both cell immobilization and iron bio-oxidation. in flask experiments, five successive batch colonization cycles resulted in 6-fold increase in ferrous iron bio-oxidation rate. evaluation of immobilized cells activity through two approaches as wet and dry storage demonstrated the higher activity of wet stored bacteria than dry ones after a period of two months. the dry stored bacteria lost almost all their activities after eight weeks. whereas, the activity of wet stored samples was 30% at the same time. in bioreactor experiments, use of a packed-bed bioreactor with hemp fibres, the maximum ferrous iron bio-oxidation rate of 2.46 kg m^-3 h^-1 was obtained at dilution rate of 3.97 h^-1, and retention time of 0.25 h. our experimental results could pave a new platform for the application of hemp fibre as an inexpensive and environmentally friendly support material in bacterial cell immobilization, especially in ferrous iron bio-oxidation process using acidophilic bacteria.
|
کلیدواژه
|
Acidithiobacillus Ferrooxidans ,Bio-Oxidation ,Hemp Fibres ,Immobilization ,Packed-Bed Bioreactor
|
آدرس
|
Noshirvani University Of Technology, Faculty Of Chemical Engineering, Biotechnology Research Laboratory, Iran
|
پست الکترونیکی
|
nedaakhlaghi4@gmail.com
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|