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   Hemp Fibres As Novel Green Support Material For Immobilization of Acidithiobacillus Ferrooxidans  
نویسنده Akhlaghi N.
منبع International Journal Of Engineering - 2019 - دوره : 32 - شماره : 9 - صفحه:1225 -1230
چکیده    The suitability of hemp fibres as green support material for immobilization of acidithiobacillus ferrooxidans cells in ferrous iron bio-oxidation process was investigated. a series of experiments were carried out in shake flasks and bioreactor for both cell immobilization and iron bio-oxidation. in flask experiments, five successive batch colonization cycles resulted in 6-fold increase in ferrous iron bio-oxidation rate. evaluation of immobilized cells activity through two approaches as wet and dry storage demonstrated the higher activity of wet stored bacteria than dry ones after a period of two months. the dry stored bacteria lost almost all their activities after eight weeks. whereas, the activity of wet stored samples was 30% at the same time. in bioreactor experiments, use of a packed-bed bioreactor with hemp fibres, the maximum ferrous iron bio-oxidation rate of 2.46 kg m^-3 h^-1 was obtained at dilution rate of 3.97 h^-1, and retention time of 0.25 h. our experimental results could pave a new platform for the application of hemp fibre as an inexpensive and environmentally friendly support material in bacterial cell immobilization, especially in ferrous iron bio-oxidation process using acidophilic bacteria.
کلیدواژه Acidithiobacillus Ferrooxidans ,Bio-Oxidation ,Hemp Fibres ,Immobilization ,Packed-Bed Bioreactor
آدرس Noshirvani University Of Technology, Faculty Of Chemical Engineering, Biotechnology Research Laboratory, Iran
پست الکترونیکی nedaakhlaghi4@gmail.com

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