Gadolinium-Hematoporphyrin: new potential MRI contrast agent for detection of breast cancer cell line (MCF-7)

Gadolinium-porphyrins have been synthesized and are currently being investigated as magnetic resonanceimaging (mri) contrast agents . this study aimed to synthesize gd-hematoporphyrin and applicate it for in vitrodetection of breast cancer cell line (mcf-7).methods: the naturally occurring porphyrin (hematoporphyrin) was inserted with gadolinium (iii) nitrate hexahydrateto yield gd-h. t1 relaxation times and signal enhancement of the contrast agents were presented, and the results werecompared. uv spectrophotometer measured the attachment of gd to the cell membrane ofmcf-7.results: most of gadolinium chloride (gdc13) was found in the washing solution, indicate that it didn 't fixed to thebreast cell membranes during incubation. gd-dtpa showed some uptake into the mcf-7 cell membranes with incubation,however, its uptak e was significantly lower than gd-h.conclusion: good cell memberan uptake of gd-porphyrin is comparable to controls, indicating selective delivery it tothe breast cell line and considerable potency in diagnostic mr imaging for detection of breast cancer.