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   Interferon‑induced protein 44‑like gene promoter is differentially methylated in peripheral blood mononuclear cells of systemic lupus erythematosus patients  
   
نویسنده karimifar mansour ,pakzad bahram ,karimzadeh hadi ,mousavi maryam ,kazemi mehdi ,salehi amirhossein ,vatandoust nasimeh ,amini guilda ,akbari mojtaba ,salehi rasoul
منبع journal of research in medical sciences - 2019 - دوره : 24 - شماره : 11 - صفحه:1 -7
چکیده    Background: the objectives of this study were to compare the interferon‑induced protein 44‑like (ifi44l) promoter methylation level between systemic lupus erythematosus (sle) patients and healthy controls and to evaluate its diagnostic value in sle. materials and methods: the ifi44l promoter methylation level was measured in 49 patients with sle and 50 healthy controls. quantitative analysis of promoter methylation ifi44l gene in genomic dna samples extracted from peripheral blood mononuclear cells was examined in sle patients and healthy controls. the level of dna methylation was compared between sle patients and healthy controls as well as within sle patient groups based on the presence of renal involvement. moreover, diagnostic values of ifi44l were calculated. results: the ifi44l promoter methylation level in sle patients was significantly lower than healthy controls (median, 43.8 vs. 57, respectively; p = 0.008). the level of ifi44l promoter methylation was not significantly different between sle patients with renal involvement and sle patients without renal involvement (84.6% vs. 92.7%, respectively; p = 0.774). the ifi44l promoter methylation level ≤94.3% was the best cutoff point with a sensitivity of 91.8% and a specificity of 38% to distinguish patients with sle from healthy individuals. conclusion: the level of ifi44l promoter methylation from whole peripheral blood in iranian sle patients was significantly lower than healthy controls. furthermore, the dna methylation level of ifi44l promoter was not associated with renal damage in patients with sle.
کلیدواژه Autoimmune diseases ,DNA methylation ,interferon‑induced protein 44‑like ,systemic lupus erythematosus
آدرس isfahan university of medical sciences, isfahan metabolic bone disorders research center, Iran, isfahan university of medical sciences, isfahan metabolic bone disorders research center, Iran, isfahan university of medical sciences, isfahan metabolic bone disorders research center, Iran, isfahan university of medical sciences, isfahan metabolic bone disorders research center, Iran, isfahan university of medical sciences, isfahan metabolic bone disorders research center, Iran, isfahan university of medical sciences, isfahan metabolic bone disorders research center, Iran, isfahan university of medical sciences, school of medicine, department of genetics and molecular biology, Iran, isfahan university of medical sciences, school of medicine, department of genetics and molecular biology, Iran, isfahan university of medical sciences, isfahan endocrine and metabolism research center, Iran, isfahan university of medical sciences, isfahan metabolic bone disorders research center, Iran
 
     
   
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