Genotyping of peroxisome proliferator-activated receptor gamma (PPAR-gamma) polymorphism (Pro12Ala) in Iranian population

Background: the peroxisome proliferator-activated receptor-γ (ppar-γ) is a nuclear hormone receptor. it is predominantly expressed in adipose tissue and as a receptor for thiazolidinediones, it has drawn attentions towards itself as a key molecule to trigger pathways involving in some diseases such as cancers, type 2 diabetes, inflammations and osteoporosis. a proline changed to alanine in codon 12 of ppar-γ gene (pro12ala) has been known to be responsible for decreased risk of type 2 diabetes. the aim of the present study is to investigate the frequency of pro12ala polymorphism in ppar-γ in healthy iranian population to compare with other populations. understanding this polymorphism may help us in better diagnosis, prevention, and therapeutic approaches toward a better management of diseases such as type 2 diabetes and osteoporosis. methods: 128 healthy volunteers were enrolled in this study. to determine single nucleotide polymorphisms (snps), we did real time polymerase chain reaction (rt-pcr), using taqman allelic discrimination assays. results: genotype frequencies for ppar-γ gene pro12ala (rs1801282) polymorphism were 0.86 for cc, 0.14 for cg, 0.00 for gg while allelic frequencies were 0.93 and 0.0.07 for c and g, respectively. conclusions: there are statistical differences between the distribution of the ppar-γ-2 pro12ala polymorphism in other populations and iranian population.