مطالعه اثر توام تحریک الکتریکی با فرکانس پائین و کاربامازپین بر شاخصهای رفتارca1 حرکتی و تعادلی در طی کیندلینگ یک طرفه در ناحیه 1 هیپوکامپ پشتی در موشهای صحرایی نر بالغ

ﻣﻘﺪﻣﻪ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺑﻪ ﺑﺮرﺳﯽ اﺛﺮات ﺗﻮام ﺗﺤﺮﯾﮏ اﻟﮑﺘﺮﯾﮑﯽ ﺑﺎ ﻓﺮﮐﺎﻧﺲ ﭘﺎﯾﯿﻦ lfs و ﮐﺎرﺑﺎﻣﺎزﭘﯿﻦ cbz ، در ﻃﯽ ﺻﺮع زاﺋﯽ ﻫﯿﭙﻮﮐﺎﻣﭗ ﭘﺸﺘﯽ ﺑﺎ روش ﮐﯿﻨﺪﻟﯿﻨﮓ اﻟﮑﺘﺮﯾﮑﯽ k ، ﺑﺮ ﺷﺎﺧﺺﻫﺎی رﻓﺘﺎر ﺣﺮﮐﺘﯽ و ﺗﻌﺎدﻟﯽ ﻣﻮشﻫﺎی ﺻﺤﺮاﺋﯽ ﻧﺮ ﺑﺎﻟﻎ ﭘﺮداﺧﺘﻪ ﺷﺪ. روش ﮐﺎر: اﯾﻦ ﻣﻄﺎﻟﻌﻪ از ﻧﻮع ﺗﺠﺮﺑﯽ ﺑﻮد و ﺑﺮای اﺟﺮای آن ﺗﻌﺪاد 56 ﺳﺮ ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﺑﺎﻟﻎ ﻧﮋاد وﯾﺴﺘﺎر ﺑﻪ ﻃﻮر ﺗﺼﺎدﻓﯽ ﺑﻪ 8 ﮔﺮوه ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﺗﺤﺮﯾﮑﺎت ﮐﯿﻨﺪﻟﯿﻨﮓ ﺑﻪ روش ﺳﺮﯾﻊ در ﻧﺎﺣﯿﻪ ﻫﯿﭙﻮﮐﺎﻣﭗ ﭘﺸﺘﯽ اﻧﺠﺎم ﺷﺪ. در ﮔﺮوهﻫﺎی دﯾﮕﺮ، ﺗﺰرﯾﻖ ﮐﺎرﺑﺎﻣﺎزﭘﯿﻦ ﻗﺒﻞ از ﮐﯿﻨﺪﻟﯿﻨﮓ اﻧﺠﺎم ﻣﯽﺷﺪ. ﺗﺤﺮﯾﮑﺎت lfs ﻧﯿﺰ ﺑﻼﻓﺎﺻﻠﻪ ﺑﻌﺪ از اﻋﻤﺎل ﮐﯿﻨﺪﻟﯿﻨﮓ در ﮔﺮوهﻫﺎی ﻣﻮرد ﻧﻈﺮ اﻧﺠﺎم ﻣﯽﺷﺪ. ﺷﺎﺧﺺﻫﺎی رﻓﺘﺎر ﺣﺮﮐﺘﯽ و ﺗﻌﺎدل ﺣﯿﻮاﻧﺎت ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮنﻫﺎی ﻣﯿﺪان ﺑﺎز و ﻣﯿﻠﻪ ﭼﺮﺧﺎن اﻧﺪازهﮔﯿﺮی ﻣﯽﺷﺪﻧﺪ. ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ دادهﻫﺎی رﻓﺘﺎری ﺑﺎ اﺳﺘﻔﺎده آزﻣﻮنﻫﺎی آﻧﺎﻟﯿﺰ وارﯾﺎﻧﺲ و ﮐﺮوﺳﮑﺎل-واﻟﯿﺲ اﻧﺠﺎم ﺷﺪﻧﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺗﻮاﻧﺎﺋﯽ ﺣﻔﻆ ﺗﻌﺎدل در آزﻣﻮن ﻣﯿﻠﻪ ﭼﺮﺧﺎن در ﮔﺮوهﻫﺎی cbz20+k+lfs و cbz40+k+lfs ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﯿﻨﺪل و k+lfs اﻓﺰاﯾﺶ ﻣﻌﻨﯽداری داﺷﺘﻪ اﺳﺖ. ﺗﺤﻠﯿﻞ ﻧﺘﺎﯾﺞ رﻓﺘﺎر ﺣﺮﮐﺘﯽ ﻣﻮشﻫﺎی ﮐﯿﻨﺪﻟﯽ ﻧﺸﺎن داد ﮐﻪ ﻣﯿﺰان رﻓﺘﺎر ﺟﺴﺘﺠﻮﮔﺮاﻧﻪ ﮔﺮوهﻫﺎی درﯾﺎﻓﺖ ﮐﻨﻨﺪه دارو و lfs ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه k+lfs و k ﺑﻪ ﺻﻮرت ﻣﻌﻨﯽداری اﻓﺰاﯾﺶ ﯾﺎﻓﺘﻪ اﺳﺖ. ﻫﻤﭽﻨﯿﻦ، در ﺑﺮرﺳﯽ ﺷﺎﺧﺺ اﯾﺴﺘﺎدن دوﭘﺎﺋﯽ ﮔﺮوهﻫﺎی ﺗﺮﮐﯿﺒﯽ ﻧﺴﺒﺖ ﺑﻪ k+lfs و ﮔﺮوهﻫﺎی درﯾﺎﻓﺖﮐﻨﻨﺪه دارو ﮐﺎﻫﺶ ﻣﻌﻨﯽداری ﻧﺸﺎن دادﻧﺪ. ﻧﺘﯿﺠﻪ ﮔﯿﺮی: ﻣﺪت زﻣﺎن ﺣﻔﻆ ﺗﻌﺎدل ﺑﺮ روی ﻣﯿﻠﻪ ﭼﺮﺧﺎن در ﮔﺮوهﻫﺎی درﯾﺎﻓﺖ ﮐﻨﻨﺪه دارو و lfs ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﯿﻨﺪل و k+lfs اﻓﺰاﯾﺶ ﻣﻌﻨﯽداری را ﻧﺸﺎن داد 0/05 < p . ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦ ﺷﺎﺧﺺ ﺗﻌﺪاد اﯾﺴﺘﺎدن دوﭘﺎﺋﯽ، ﺑﯿﺎﻧﮕﺮ ﮐﺎﻫﺶ ﻣﻌﻨﯽدار ﮔﺮوهﻫﺎی درﯾﺎﻓﺖﮐﻨﻨﺪه دارو و lfs ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﯿﻨﺪل و k+lfs اﺳﺖ 0/05 < p .

study of the combined effect of low frequency stimulation and carbamazepine on motor and balance behavior indices during unilateral kindling in the ca1 region of the dorsal hippocampus in adult male rats

introduction: in this study, the combined effects of low frequency stimulation lfs and carbamazepine cbz were investigated on the motor behavior and balance indices of adult male rats during dorsal hippocampal epilepsy by electrical kindling method.method: in this experimental study, 56 adult male wistar rats were randomly divided into 8 groups. kindling stimulations were performed rapidly in the dorsal hippocampus. in other groups, carbamazepine was injected before kindling. lfs stimulation was also performed immediately after kindling in the target groups. the animals’ motor behavior and balance indices were measured using open field and rotating bar tests. behavioral data analysis was performed using analysis of variance and kruskal-wallis tests.results: the results showed that the ability to maintain balance in the rotating rod test in the cbz20+k+lfs and cbz40+k+lfs groups had a significant increase compared to the kindle and k+lfs groups. analysis of the motor behavior of kindley mice showed that the search behavior of the drug and lfs groups has significantly increased compared to the k+lfs and k groups. also, in the study of bipedal standing index, the combined groups showed a significant decrease compared to k+lfs and drug-receiving groups.conclusion: the duration of balance on the rotating bar in the drug and lfs groups showed a significant increase compared to the kindle and k+lfs groups p <0.05 . comparison of the mean number of bipedal standing indices indicated significant decrease in the drug and lfs groups compared to the kindle and k+lfs groups p <0.05 .