بررسی اثرات ضد اضطرابی عصاره متانولی گیاه ازمک (lepidium draba l) به روش ماز صلیبی شکل در موش سوری

ﻣﻘﺪﻣﻪ: ﮔﯿﺎه ازﻣﮏ lepidium draba l ﯾﮑﯽ از ﺑﺎ ارزشﺗﺮﯾﻦ ﮔﯿﺎﻫﺎن داروﯾﯽ ﺧﺎﻧﻮاده ﺷﺐ ﺑﻮ اﺳﺖ. ﻫﺪف از اﻧﺠﺎم اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﯽ اﺛﺮات ﺿﺪ اﺿﻄﺮاﺑﯽ ﻋﺼﺎره اﺗﺎﻧﻮﻟﯽ ﮔﯿﺎه ازﻣﮏ ﺑﺎ دوزﻫﺎی ﻣﺨﺘﻠﻒ و ﻧﯿﺰ ﺑﺮرﺳﯽ ﻧﻘﺶ ﮔﯿﺮﻧﺪهﻫﺎی ﺑﻨﺰودﯾﺎزﭘﯿﻨﯽ ﺑﺮ اﺛﺮ ﺿﺪ اﺿﻄﺮاﺑﯽ ﮔﯿﺎه ﻣﺬﮐﻮر در ﻣﻮش ﺳﻮری ﻣﯽﺑﺎﺷﺪ. روش ﮐﺎر: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ از 48 ﺳﺮ ﻣﻮش ﺳﻮری ﻧﺮ ﺑﻪ وزن 25-30 ﮔﺮم اﺳﺘﻔﺎده ﺷﺪ. ﻣﻮشﻫﺎی اﻧﺘﺨﺎب ﺷﺪه ﺑﻪ 6 ﮔﺮوه 8 ﺗﺎﯾﯽ ﺑﻪ ﻃﻮر ﺗﺼﺎدﻓﯽ ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﺑﺮای ﺑﺮرﺳﯽ اﺛﺮات ﺿﺪ اﺿﻄﺮاﺑﯽ ﻋﺼﺎره ﻣﺘﺎﻧﻮﻟﯽ ﮔﯿﺎه 50، 100 و 200 ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم وزن ﺑﺪن ، ﺳﺎﻟﯿﻦ ﺑﺎ ﺣﺠﻢ ﺑﺮاﺑﺮ ﺑﻪ ﻋﻨﻮان ﮐﻨﺘﺮل ﻣﻨﻔﯽ، دﯾﺎزﭘﺎم 2mg/kg ﺑﻪ ﻋﻨﻮان ﮐﻨﺘﺮل ﻣﺜﺒﺖ و ﻓﻠﻮﻣﺎزﻧﯿﻞ 2mg/kg ﺑﻪ ﻫﻤﺮاه دوز ﺑﺎﻻی ﻋﺼﺎره ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﻣﮑﺎﻧﯿﺴﻢ اﺣﺘﻤﺎﻟﯽ اﺛﺮات ﺿﺪ اﺿﻄﺮاﺑﯽ ﮔﯿﺎه ﺑﻪ ﻣﻮشﻫﺎ ﺗﺰرﯾﻖ ﺷﺪ، ﺑﻌﺪ از 30 دﻗﯿﻘﻪ ﺑﻪ ﻣﺎز ﺑﻌﻼوه ای ﻣﺮﺗﻔﻊ ﻣﻨﺘﻘﻞ ﮔﺮدﯾﺪﻧﺪ و ﺑﻪ ﻣﺪت 5 دﻗﯿﻘﻪ ﺷﺎﺧﺺﻫﺎی اﺳﺘﺎﻧﺪارد ارزﯾﺎﺑﯽ اﺿﻄﺮاب از ﻃﺮﯾﻖ ﻣﺸﺎﻫﺪه در آﻧﻬﺎ ﺑﺮرﺳﯽ و ﺛﺒﺖ ﺷﺪ. ﺑﺮای آﻧﺎﻟﯿﺰ دادهﻫﺎ از ﻧﺮم اﻓﺰار instat اﺳﺘﻔﺎده ﺷﺪ. ﻧﺘﺎﯾﺞ ﺑﻪ ﺻﻮرت mean ± sem ﮔﺰارش ﮔﺮدﯾﺪ و 0/05 < p ﻣﻌﻨﺎدار ﺗﻠﻘﯽ ﺷﺪه اﺳﺖ. ﯾﺎﻓﺘﻪﻫﺎ: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﻋﺼﺎره ﻣﺘﺎﻧﻮﻟﯽ ﮔﯿﺎه ازﻣﮏ ﺑﻪ ﺻﻮرت واﺑﺴﺘﻪ ﺑﻪ دوز ﻣﻮﺟﺐ اﻓﺰاﯾﺶ ﺗﻌﺪاد ورود و ﻣﺪت زﻣﺎن اﻗﺎﻣﺖ ﺑﻪ ﺑﺎزوﻫﺎی ﺑﺎز ﮔﺮدﯾﺪ. ﻧﺘﯿﺠﻪ ﮔﯿﺮی: اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن ﻣﯽدﻫﺪ ﮐﻪ ﮔﯿﺎه ازﻣﮏ ﻣﯽﺗﻮاﻧﺪ در درﻣﺎن اﺿﻄﺮاب ﻣﺆﺛﺮ ﺑﺎﺷﺪ ﮐﻪ اﯾﻦ اﺛﺮ اﺣﺘﻤﺎﻻً از ﻃﺮﯾﻖ ﺳﯿﺴﺘﻢ ﮔﺎﺑﺎ ارژﯾﮏ ﺻﻮرت ﻣﯽﭘﺬﯾﺮد.

evaluation of anxiolytic effects of methanolic extract of lepidium draba l by cross-maze method in mice

introduction: lepidium draba l l. draba is one of the most valuable medicinal plants belonging to the nightshade family. this study was designed to evaluate anxiolytic effects of different doses of the methanolic extract of l. draba and also the role of benzodiazepine receptors.method: in this study, 48 male mice weighing 25-30 g were used. the selected mice were randomly divided into 6 groups of 8. to evaluate the anxiolytic effects of methanolic extract of l. draba 50, 100 and 200 mg/kg , saline with equal volume as negative control, diazepam 2 mg/kg as positive control and flumazenil 2 mg/kg with high dose of extract was injected into mice to investigate the possible mechanism of the plant’s anxiolytic effects, after 30 minutes they were transferred to elevated plus maze and for 5 minutes the standard indicators of anxiety assessment were observed and recorded by observing them. instat software was used to analyze the data. the results were reported as mean ± sem and p <0.05 was considered significant.results: the results showed that methanolic extract of plant increased the number of entry and length of stay in the open arms in a dose-dependent manner.conclusion: this study shows that l. draba plant can be effective in treating anxiety probably through gabaergic system.