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   Activation of apoptotic cell death by skin mucus from Asian swamp eel (Monopterus albus) against human lung cancer cell line  
   
نویسنده hilles ayah rebhi ,mahmood syed ,kaderi mohd arifin ,hashim ridzwan
منبع journal of agricultural and marine sciences - 2019 - دوره : 24 - شماره : 1 - صفحه:39 -43
چکیده    Asian swamp eel (monopterus albus) is a freshwater fish which distributed widely in the east of india mainly across malay peninsula and indochinese peninsula, it is also broadly distributed in the southern areas of east asia including, southeastern china, western japanese archipelago, and korean peninsula. it lives in muddy places, rice paddies, and slow-flowing currents areas. it has a unique morphological elongated body which is similar to snake and covered with a thick layer of mucus. the objective of this study is to screen the cytotoxic activity of eel skin mucus extracts and to evaluate the mechanism of cell death apoptosis or necrosis based on caspases activity assays. for this purpose eel skin mucus was collected from healthy fresh eels to prepare aqueous and methanol extracts. preliminary cytotoxicity study was demonstrated against non-small-cell lung carcinoma cell line (a549) using cell viability assay which revealed that methanol extract was more potent than aqueous extract based on the response of esm methanol and aqueous extracts to the relevant ic_50 concentrations as ic_50 values were 621±0.09 μg/ml and 845 ± 0.25 μg/ml respectively. subsequently cell death was induced through triggering apoptosis by the activation of caspase-3/7, 8 and 9. in conclusion, the present study has revealed the cytotoxic potentials of eel skin mucus which may lead to the development of new anticancer agents.
کلیدواژه Monopterus albus; cytotoxic activity; apoptosis; caspases
آدرس international islamic university malaysia, kulliyyah of allied health sciences, department of biomedical sciences, Malaysia, university malaysia pahang, faculty of engineering technology, centre of excellence for advanced research in fluid flow (cariff), department of pharmaceutical engineering, Malaysia, international islamic university malaysia, kulliyyah of allied health sciences, department of biomedical sciences, Malaysia, international islamic university malaysia, kulliyyah of allied health sciences, department of biomedical sciences, Malaysia
 
     
   
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