ارزیابی تاثیرات تنظیم‌کننده‌های مسیر pi3k بر جنین‌های متوقف‌شده انسانی نوع یک در آزمایشگاه

هدف: یکی از چالش‌های لقاح آزمایشگاهی، توقف تکوین جنین پیش از لانه‌گزینی است. هدف از این مطالعه ارزیابی تاثیرات تنظیم کننده‌های مسیر pi3k  بر از سرگیری تکوین جنین‌های متوقف‌شده انسانی نوع یک در شرایط آزمایشگاهی است. مواد و روش‌ها: در این مطالعه، با رضایت آگاهانه بیماران، از جنین‌های 2 تا 3 سلولی متوقف‌شده‌ی 72 ساعته انسانی بخش جنین‌شناسی پژوهشگاه رویان، استفاده شد. گروه‌های مطالعه، شامل کنترل، chir99021 (1 میکرومولار)، its (0.5 درصد) و e8 (0.1 درصد + 10 درصد سرم) بود. محیط های کشت، به مدت 4 ساعت انکوبه شدند و سپس جنین‌ها به طور تصادفی به گروه‌ها منتقل و به مدت 48 تا 72 ساعت کشت داده شدند. درنهایت مورفولوژی جنین‌ها توسط میکروسکوپ اینورت ارزیابی شد. داده ها با نرم‌افزار spss ارزیابی و آستانه معنی داری p<0.05 اتخاذ ‌شد. نتایج: نرخ توقف در گروه‌های chir99021 و its، نسبت به گروه کنترل، کاهش معنیداری پیدا کرد. همچنین نرخ تکوین تا مرحله پیش از مورولا در هر سه گروه آزمایش نسبت به گروه کنترل، افزایش معنی داری یافت. در حالیکه در گروه کنترل هیچ یک از جنین‌ها به مرحله بلاستوسیست نرسیدند، در هر یک از گروه‌های chir99021 و its، دو جنین تا مرحله بلاستوسیست پیشرفت کردند. نتیجه‌گیری: یافته‌های ما نشان داد که its و chir99021 با تنظیم مسیر pi3k در القای چرخه‌سلولی جنین‌های متوقف‌شده نوع یک، تاثیر به‫سزایی دارند.

evaluation of the effects of pi3k pathway regulators on human arrested type i embryos in vitro

aim: infertility is a complex issue that affects many couples worldwide. in vitro fertilization (ivf) has been a groundbreaking technique in addressing this challenge, but it comes with its own set of problems in the development of embryos during the pre-implantation stages. understanding the processes involved in embryo development is crucial in overcoming these hurdles. during the pre-implantation stage, one of the challenges of treating infertility through in vitro fertilization is the developmental arrest of the embryo. in embryonic arrest, cell division stops for at least 24 hours, which, if it occurs in infertility treatments, leads to failure in art cycles.cell signaling pathways play a vital role in the development and progression of embryos. the phosphatidylinositol 3-kinase (pi3k) pathway, known for its efficiency in regulating the cell cycle and various cellular processes, was the aim of this study. by targeting this pathway, we aimed to explore the effects of specific factors - its (insulin, transferrin, selenium), essential e8 medium (e8), and the chir99021 small molecule - on the resumption of development in type i arrested human embryos under in vitro conditions.material and methods: in this study, firstly, after receiving ethics approval and patient consent, day 3 embryos (2-3 cell stage) from the embryology department of royan research institute were utilized. the experimental groups consisted of control, chir99021, its, and e8. the optimum concentrations were chosen 1 for chir99021, 0.5% for its, and 0.1% for e8 + 10% serum. the culture medium for these groups was prepared and covered with liquid paraffin before being incubated at 37°c and 5% co2 for 4 hours. subsequently, the embryos were transferred randomly to either experimental or control groups and cultured in an incubator for 48 to 72 hours. morphological evaluations of the embryos were conducted using an inverted microscope. data analysis was performed using spss software and chi-square test, with a significance threshold set at p<0.05.results: the findings of the study revealed that the rate of arrest in the chir99021 and its groups showed a significant reduction compared to the control group. moreover, all three experimental groups (its, chir99021, and e8) exhibited a notable increase in development rate up to the pre-morula stage when compared to the control group. interestingly, while none of the embryos in the control group progressed to the blastocyst stage, two embryos in each of the chir99021 and its groups reached this advanced developmental stage.conclusion: in conclusion, the study’s outcomes indicate the notable effect of its and chir99021 in modulating the phosphatidylinositol 3-kinase pathway to stimulate cell cycle progression in type i arrested embryos. its factor is probably able to regulate this pathway by activating insulin receptors and small molecule chir99021 by inhibiting glycogen synthase kinase 3 (gsk-3). these findings hold promise for further research and potential applications in improving the success rates of ivf treatments and addressing infertility challenges. understanding the mechanisms of embryo development is crucial for advancing reproductive medicine. it is important to note that the statistical population used in this study was limited and it is believed that more research is needed.