نانو داربست پلی کاپرولاکتان/کتیرا غنی شده با سیلیمارین به‫عنوان محافظ سلول‫های پیش ساز عصبی در شرایط تنش اکسیداتیو‬‬‬‬‬‬‬‬‬‬

هدف: مهندسی بافت به‫ روش‌هایی اطلاق می‌شود که مبتنی بر استفاده از داربست‌ها، سلول‫ها و مولکول‌های فعال بیولوژیکی برای تولید بافت‌های دارای عملکرد مشخص است. هدف آن قابلیت بازسازی، نگه‫داری و بهبود بافت آسیب‌دیده یا کل اندام است. هدف از این مطالعه تولید نانو داربست پلی کاپرولاکتان /کتیرا/سیلیمارین و بررسی زنده مانی سلول‫های pc12 بر روی داربست تحت تنش اکسیداتیو می باشد. مواد و روش‫ها: به‫منظور تهیه نانو داربست، محلول پلی کاپرولاکتان 7 درصد (حل شده در استیک اسید)، محلول کتیرا 7/0 درصد وزنی (‫حل شده در استیک اسید‫) ومحلول سیلیمارین با غلظت 9/0 درصد وزنی مخلوط شد، سپس توسط دستگاه الکتروریسی داربست تهیه شد. مورفولوژی داربست توسط میکروسکوپ الکترونی روبشی و ساختار شیمیایی داربست توسط طیف سنجی ftir مورد ارزیابی قرار گرفت. برای بررسی خواص آنتی اکسیدانتی داربست از گلوگز 80 میلی‫گرم بر لیتر و h2o2 ،150 مایکرو‫لیتر استفاده شد. نتایج: بررسی مورفولوژی و ساختار شیمیایی داربست، نشان دهنده تخلخل مناسب داربست پلی کاپرولاکتان /کتیرا و بارگذاری موفق سیلیمارین بر روی داربست  بود. ارزیابی خواص اکسیدانتی داربست بعد از گذشت 24 ساعت از کشت سلول‫های pc12 بر روی آن نشان دهنده افزایش زنده‫مانی سلول‫ها بر روی داربست و خواص آنتی اکسیدانتی مناسب داربست بود. نتیجه گیری: نتایج حاصل از این پژوهش نشان داد که غنی سازی داربست پلی کاپرولاکتان/کتیرا با سیلیمارین باعث افزایش توان تکثیر و زنده مانی سلول‫های pc12  تحت تنش اکسیداتیو شد. از این رو این داربست می تواند کاندیدی مناسب برای مهندسی بافت در شرایطی با تنش های اکسیداتیو بالا باشد.

polycaprolactan/ tragacanth nanoscaffold enriched with sililymarin as a protector of neural progenitor cells under oxidative stress conditions

aim: tissue engineering refers to methods that are based on the use of scaffolds, cells and biologically active molecules to produce tissues with specific functions. the purpose of tissue engineering is to build structures that can regenerate, maintain and improve damaged tissue or the whole organ. today, by using tissue engineering methods, various natural and synthetic scaffolds have been designed that can be used for nerve grafts. the physical, chemical and biological properties of the scaffold must be similar to the extracellular matrix of the body in order to avoid adhesion, growth and support the differentiation of cells. an ideal neural scaffold should have biodegradability, biocompatibility and proper tensile strength. recently, the use of polycaprolactan as a suitable biodegradable material has been evaluated in many fields of tissue engineering. antioxidants are among the substances, which seem to be able to prevent neuronal death by reducing the amount of ros. flavonoids include many compounds that have various biological effects in the body. silymarin (silybum marianum) is a flavonoid that has many effects, including anti-cancer effects and antioxidant properties. tragacanth is a known natural polymer that has excellent biological properties such as biodegradability, biocompatibility, antibacterial and wound healing ability. it is obtained from the stems and branches of the asian species tragacanth. it has outstanding structural stability against heat and acidity. the aim of this study is to produce polycaprolactan/ tragacanth /silymarin nanoscaffolds and to investigate the viability of pc12 cells on the scaffold under oxidative stress. considering that silymarin has antioxidant properties, the use of polycaprolactan/ tragacanth /silymarin nanoscaffolds can prevent neuropathy of nerve cells.material and methods: scaffolds used in this research were prepared using the electrophoretic method. for this purpose, an electrospinning machine was used, which is equipped with a rotary collector with a thickness of 70 mm and a width of 50 mm. in order to prepare a polycaprolactan/ tragacanth nanoscaffold and load silymarin on it, a 7% polycaprolactan solution (dissolved in acetic acid), 0.7% by weight tragacanth solution (dissolved in acetic acid) and 0.9% by weight silymarin solution were mixed by a magnetic stirrer for 20 minutes, and in order to make the solution uniform, sodium didecyl sulfate (sds) with a concentration 1 percent by weight of the solvent was added to the solution and the suspension was homogenized for 20 minutes with an ultrasonic device, then the scaffold was prepared by an electrospinning device. . the nanofibers were collected in a period of 6 hours, the sample collection speed was 1 ml per hour, and the nanofiber samples were collected by rotating at 250 rpm. the distance between the injection needle and the scaffold is 12 cm and this process is done at a voltage of 15 kv. the morphology of the scaffold was evaluated by scanning electron microscope (sem) and the chemical structure of the scaffold was evaluated by ftir spectroscopy. to investigate the antioxidant properties of the scaffold, glucose 80 mg/l and h2o2, 150 macro l were used.results: examining the morphology and chemical structure of the scaffold showed the proper porosity of the polycaprolactan/ tragacanth scaffold and the successful loading of silymarin on the scaffold. evaluation of the oxidant properties of the scaffold after 24 hours of pc12 cell culture on it showed the increase in cell viability on the scaffold and the appropriate antioxidant properties of the scaffold.conclusion: the results of this research showed that the enrichment of polycaprolactan/ tragacanth scaffold with silymarin increased the proliferation and survival of pc12 cells under oxidative stress. therefore, this scaffold can be a suitable candidate for tissue engineering in oxidative stress.